Composite

Part:BBa_K2082211:Design

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-08)


RFP under the control of an optimized lacZ promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 656
    Illegal AgeI site found at 768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promotor sequence was designed with the help of Badran et al. (2016)


Source

Sequence was created via gene synthesis order of IDT

References

  • Badran, A. H., Guzov, V. M. & Huai, Q. et al. (2016) Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance. Nature 533 (7601), 58–63.